User manual ZEISS UNIVERSAL R MICROSCOPE

[. . . ] All rights reserved in the event of granting of patents or registration of a utility model. Issued by: Carl Zeiss Microscopy D-07740 Jena Phone: ++49-36 41 64-1616 Telefax: ++49-36 41 64-3144 E-mail: micro@zeiss. de Internet: www. zeiss. de/micro Number of this manual: B 40-046 e Date of issue: 31. 03. 1999 0-2 B 40-046 e 03/99 Photomicrography using 35 mm SLR cameras INTRODUCTION Contents Carl Zeiss CONTENTS Page INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0-1 Title Page. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0-1 Copyright . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0-2 Table of Contents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0-3 Notes on Instrument Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0-6 Notes on Warranty. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [. . . ] · Attach camera-specific T2 adapter (2-4/3) to the relevant SLR camera housing (2-4/2). · Insert thread of objective f' = 63 mm (2-4/4) in the T2 adapter (2-4/3) attached to the SLR camera housing (2-4/2). · Remove dust cover from the 44 or 60 mm camera port (2-4/8, 11). · Insert connector for microscope camera with image position 44 mm (2-4/7) in the 44 mm camera port (2-4/8), or the connector for microscope camera with image position 60 mm (2-4/10) in the 60 mm camera port (2-4/11). · Tighten clamping screws (2-4/9, 12) to the 44 or 60 mm camera ports using the supplied ballheaded screwdriver (2-4/13). · Insert photo eyepiece S-Pl 10× / 20 (2-4/6) in the connectors for microscope cameras (24/7, 10). · Attach objective f' = 63 mm (2-4/4) with camera housing to the connectors for microscope cameras (2-4/7, 10) containing the photo eyepiece (2-4/6) until stop. · Align objective f' = 63 mm (2-4/4) with SLR camera housing in the required position and secure it by turning clamping ring (2-4/5) anticlockwise. 2-4 Attachment of 35 mm SLR cameras with objective f' = 63mm to camera ports 44 mm and 60mm 2-6 B 40-046 e 03/99 Photomicrography with 35 mm SLR cameras START-UP Attachment of 35 mm SLR cameras Carl Zeiss 2. 3. 3 Attachment of 35 mm SLR Cameras with 2. 5× / T2 Adapter to Front Port of Axiovert 25 C / CFL / CA · Remove dust cover or lens from the SLR camera housing. · Attach camera-specific T2 adapter (2-5/3) to the relevant SLR camera housing (2-5/2). · Remove dust covers from 2. 5× / T2 camera adapter for Axiovert 25 (2-5/4). The exposure time of the first photograph featuring high contrast and clearly defined object details is the border exposure time of the exposure time range for unblurred photographs. All the photographs with exposure times the border exposure time are unblurred. 2-10 B 40-046 e 03/99 Photomicrography with 35 mm SLR cameras OPERATION Contents Carl Zeiss OPERATION Contents 3 3. 1 3. 2 3. 2. 1 3. 2. 2 3. 2. 3 3. 2. 4 3. 2. 5 3. 2. 6 3. 2. 7 3. 2. 8 3. 2. 8. 1 3. 2. 8. 2 3. 2. 8. 3 3. 2. 8. 4 3. 2. 8. 5 3. 3 OPERATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2 Adaptation and Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2 Photomicrography with 35 mm SLR Cameras . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3 Photography in a Few Steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3 Setting the Color Temperature to 3200 K and Setting the Image Brightness. . . . . . . . . . . . . . . . . . . . . . 3-4 Selecting the Film . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4 Exposure Correction Depending on the Microscopy Technique. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5 Setting and Focusing of Image Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6 Exposure Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7 Trigger the Exposure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10 Further Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11 Avoid Blurs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11 Color Balance in Color Reversal Films. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11 Darkfield and Fluorescence Photography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-13 Computing the Magnification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-14 Further Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-14 Using Digital Cameras. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3 Use of bulk film . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5 Measuring range of 35 mm SLR camera, ISO 100 / 21°, camera lens f / 1. 4 with apertures k = 1. 4 - 16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8 Measuring range of 35 mm SLR camera with T2-Adapter, ISO 100 / 21° . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9 B 40-046 e 03/99 3-1 Carl Zeiss OPERATION Adaptation and functions Photomicrography with 35 mm SLR cameras 3 3. 1 OPERATION Adaptation and Functions The adaptation components for 35 mm SLR cameras for photomicrography are simple screwing and clamping adapters which are described in chapter 2, Start-up. The functions important for photomicrography with 35 mm SLR cameras (e. g. exposure programs, film insertion) are described in the operating instructions of the relevant SLR camera. [. . . ] of filters, photo reticles) Deutsches Institut für Normung (German standards association) coding system for the storage of electronically legible information (e. g. film speed) Extra-Plan (eyepiece model) Exposure Value (of light) camera lens with focal length f with aperture ratio 1 : 1. 4 focal length on the image side focal length of objective fluorescence focusing green Infinity Color-Corrected System Inverted Microscope International Organization for Standardization stop value compensation Kompens-Plan (eypiece model) reference viewing distance 250 mm metric thread, magenta microscope camera magnification of the image on 35 mm film Plan red Single Lens Reflex (camera) Sonder-Plan (photo eyepiece, projection lens for photomicrography) A-2 B 40-046 e 03/99 Photomicrography with 35 mm SLR cameras ANNEX List of abbreviations Carl Zeiss SW T2-Adapter T-function Tmax Tmin VObj Veyep. VZ W-PL Y wrench opening standard adapter for 35 mm cameras Time function (shutter remains open after first press and closes only after second press, see B-function) maximum exposure time minimum exposure time magnification of the objective magnification of the eyepiece intermediate magnification Weitfeld-Plan (wide-angle eyepiece model)) Yellow Physical and Technical Data °C hPa K kg m mm s V Centigrade Hecto-Pascal Kelvin kilogram meter millimeter second Volt B 40-046 e 03/99 A-3 Carl Zeiss ANNEX List of key words Photomicrography with 35 mm SLR cameras List of Key Words Page A adjusting aid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6 ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 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