User manual ZEISS SV11

[. . . ] All rights reserved in the event of granting of patents or registration of a utility model. Issued by: Carl Zeiss Mikroskopie D-07740 Jena Telephone: (**49) 03641 / 64-1616 Telefax: (**49) 03641 / 64-3144 Internet: mikro@zeiss. de http://www. zeiss. de Number of this manual: B 40-029 e Date of issue: 01/98 II B 40-029 e 01/98 Standard 25 ICS CONTENTS Page INTRODUCTION. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I Title page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I Copyright. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . II Contents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . III Notes on instrument safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [. . . ] We would recommend you to keep the packaging material so that the instrument can be stored for a longer period of time or returned to the manufacturer. 2. 1 Unpacking the instrument Remove the microscope from the transport case and place it on the worktable. 2. 2 Attach binocular tube 45°/20 ICS or binocular phototube 35°/20 ICS. Loosen hexagonal screw (2-1/5) using the SW3 ball-headed screwdriver (2-1/4). Remove dust covers (2-1/1) from tube underside and the dovetail mount on the stand. Hold the binocular tube (2-1/3) or the binocular phototube 2-1/2) in a slightly inclined position and attach it to the stand mount via the dovetail. Turn the binocular tube into the required observation position and tighten the hexagonal screw using the screwdriver. 2-1 Attachment of binocular tubes Remove dust caps (2-2/2) according to the number of objectives and screw objectives (22/3) in nosepiece (2-2/1) clockwise one by one, starting with the lowest magnification. The dust caps should remain on those nosepiece eyes which are not required. Fig. 2-2 Screwing-in of objectives B 40-029 e 01/98 2-3 Standard 25 ICS 2. 4 Insertion of eyepieces Remove both protection caps (2-3/1) from the binocular tube (2-3/2). Insert the fixed eyepiece, e. g. The focusing eyepiece is used to compensate for ametropia of the eyes. Fig. 2-3 Insertion of eyepieces 2. 4. 1 Insertion of eyepiece reticle The eyepieces Pl 10x/18 Br. The slight image shift caused by the additional path through the glass is taken into account on the diopter scale by the fact that the zero point position is indicated not by the white dot W (24/W) but by the red dot R (2-4/R). 3-13 Attachment of adapters for video cameras . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-14 Insertion of 8x drawing eyepiece . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2 Fig 3-3 Fig 3-4 Fig 3-5 Fig 3-6 Fig 3-7 Fig 3-8 Fig 3-9 Fig 3-10 Fig 3-11 Fig 3-12 Fig 3-13 Switch on the instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3 Insertion of filters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3 Setting of brightfield. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4 Setting of interpupillary distance of binocular tube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4 Setting of diaphragm images according to KÖHLER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5 Setting of phase contrast using condenser 0. 9 Z . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6 Centering of phase stops in phase contrast . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7 Setting of phase contrast using Ph condenser II Z 0. 9. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7 Setting of polarization contrast . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9 Measurement of lengths. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10 Attachment of SLR camera, e. g. CONTAX 167 MT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11 Attachment of MC 80 DX microscope camera. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-13 Insertion of 8x drawing eyepiece . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-16 B 40-029 e 01/98 3-1 Standard 25 ICS 3-2 B 40-029 e 01/98 Standard 25 ICS 3 3. 1 OPERATION Switch on the instrument Switch on the instrument via the on/off switch (3-1/3) on the instrument rear. The green LED (3-1/2) must light up. Set the required brightness via the "Brightness" control (3-1/1). Fig. 3-1 Switch on the instrument Depending on the application, place one or several of the following dia. 32 filters (3-2/1) on the dustprotection glass of the luminous-field diaphragm: interference wide-band filter, green, 32x4, for contrast enhancement in b/w photography of stained sections and for phase contrast; CB 3 conversion filter, 32x2, to generate the correct color temperature of 3200 K when artificial light color reversal film is used. CB 3, 32x2, and CB 12, 32x2, conversion filters for use with daylight color film Fig. [. . . ] mount, see page 3-3. 4-6 B 40-029 e 01/98 Standard 25 ICS Problem Low resolving power and poor image contrast Cause Aperture diaphragm opening not set correctly. Remedy Set aperture diaphragm opening in accordance with the 2/3 rule or depending on the specimen features, see page 3-5. Focus condenser and set condenser lens 0. 9 correctly or switch it off, see pages 3-4, 3-5. Use the correct 0. 17 mm cover slips Condenser not focused correctly and condenser lens 0. 9 not switched correctly. Wrong cover slip thickness for 0. 17 transmitted light objectives Use of no or unspecified immersion oil with CZ immersion objectives Air bubbles in the immersion oil Use CZ immersion oil 518 N, see page 4-10. [. . . ]