User manual ZEISS STANDARD 25 ICS

[. . . ] Standard 25 ICS Transmitted Light Microscope Operating Manual Standard 25 ICS Knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument. The specifications are subject to change; the manual is not covered by an update service. © Unless expressly authorized, forwarding and duplication of this document, and the utilization and communication of its contents are not permitted. [. . . ] foc. , to enable compensation of ametropia. Use the eyelens of the focusing eyepiece to focus on the line figure of the eyepiece reticle; focus on the edge of the field of view if no eyepiece reticle is used. Focus on the microscope image of a specimen via the focusing drive by looking through the eyepiece with reticle. When the image and the eypiece reticle are in focus in the above eyepiece, focus the image for the second eye via the focusing eyelens of the second eyepiece. The position of the focusing drive on the stand must not be changed. 2. 5 Setting of interpupillary distance and viewing height The eyepiece distance is matched to the individual interpupillary distance by swinging the eyepiece tubes symmetrically towards one another. The viewing height can be increased (2-5/A) or reduced (2-5/B) by turning the entire binocular tube through 180°. Fig. 2-5 Setting the interpupillary distance and viewing height of the binocular tube B 40-029 e 01/98 2-5 Standard 25 ICS 2. 6 Attachment of specimen holder to mechanical stage 75x30 R The Standard 25 ICS features the mechanical stage 75x30 R with ceramic coating (2-6/6). The specimen is precisely moved in x and y using the user-friendly coaxial drive (2-6/4). The x/y gradation on the stage surface (2-6/5) with the two vernier scales helps to relocate certain specimen spots. The readily mounted specimen holder with spring clip R (453536) (2-6/1) is part of the standard configuration. As an alternative, it is also possible to use - the specimen holder with sprig clip R (473448) (2-6/2) or - the specimen holder for one-handed operation (453548) (2-6/3). Here, the microscope slide (2-6/7) is inserted into the specimen holder along the guiding edge, the spring clip is swung out and will automatically clamp the microscope slide in its stop position. The specimen holders can be easily exchanged Fig. 2-6 Attachment of specimen holder to mechanical stage 75x30 R in the x-direction using the two fixation screws on the guiding rail. Loosen the two fixation screws, pull out specimen holder to the front and insert new specimen holder until it engages in the guiding slots. Tighten the two fixation screws again. 2-6 B 40-029 e 01/98 Standard 25 ICS 2. 7 2. 7. 1 Attachment of condensers Attach condenser 0. 9 Z Unscrew both centering screws (2-7/3) on the condenser carrier (2-7/2) until the condenser 0. 9 Z (2-7/9) can be easily inserted in the condenser carrier. Insert condenser 0. 9 Z (2-7/9) in the condenser carrier (2-7/2) in such a way that, firstly, the dovetail of the condenser is pressed against spring pin (2-7/1) in the condenser carrier and, secondly, the aperture diaphragm lever (2-7/8) can be conveniently operated from the front. Tighten both centering screws (2-7/3) on the condenser carrier (2-7/2) until they engage in the dovetail and keep the condenser (2-7/9) in position. The Ph 2 phase stop carrier (2-7/4) must be mounted as follows: Screw locking screw (2-7/6) into the left drilled hole (2-7/7) on the underside of the condenser carrier. The locking screw keeps the Ph 2 phase stop carrier in its functional position. Then screw Ph 2 phase stop carrier (2-7/4) into the drilled hole (2-7/5) on the underside of the condenser carrier. Fig. 2-7 Attachment of condenser 0. 9 Z 2. 7. 2 Attach phase contrast condenser II Z 0, 9 Ph 1, 2, 3 and D (0. 6/0. 9) With the exception of the orientation in the condenser carrier (2-7/2), the phase contrast condenser II Z 0. 9 is inserted in the Standard 25 ICS in the same way as the above condenser 0. 9 Z. Insert condenser II Z 0. 9 (2-8/1) in the condenser carrier (2-7/2) in such a way that knob (2-8/2) points to the observer. Fig. 2-8 Attachment of phase contrast condenser II Z 0. 9 Ph 1, 2, 3 and D (0. 6/0. 9) B 40-029 e 01/98 2-7 Standard 25 ICS 2. 8 Connecting the instrument to the line Check whether the voltage indicated at the rear of the instrument complies with the line voltage!Do not connect the instrument to the line if the instrument voltage and the line voltage are not identical and make sure to inform the nearest CZ agency or CZ service agency. Connect the line cable with connector (2-9/4) to the instrument socket (2-9/3) and connect the earth- contact plug (2-9/5) to the line. Switch on the instrument via the on/off switch (2-9/2) on the instrument rear. The green LED (2-9/1) lights up to indicate that the instrument is ready for operation (on/off switch in "I" position), and the integrated halogen lamp must also be on. Fig. 2-9 Connecting the instrument to the line 2-8 B 40-029 e 01/98 Standard 25 ICS OPERATION Contents 3 3. 1 3. 2 3. 3 3. 4 3. 5 3. 6 3. 7 3. 7. 1 3. 7. 2 3. 8 3. 9 OPERATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3 Switch on the instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3 Setting of transmitted-light brightfield for KÖHLER illumination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4 Setting of transmitted-light phase contrast . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6 Setting of transmitted-light darkfield. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8 Setting of transmitted light polarization contrast . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9 Measurement of lengths . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10 Attachment of photomicrography equipment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . CONTAX 167 MT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11 Attachment of MC 80 DX microscope camera (35 mm film cassette) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-13 Attachment of adapters for video cameras . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-14 Insertion of 8x drawing eyepiece . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [. . . ] Apply oil again to remove the bubbles. Immersion oil at the front lens of a Clean the front lens of the dry dry objective objective, see page 4-3. ring to the correct thickness, see page 1-6. Dirt or dust on the optical surfaces Clean the appropriate of objectives, eyepieces, components, see page 4-3 condensers or filters Image aberration Condenser not set correctly. Nosepiece not correctly switched to stop position Specimen is not fixed on the mechanical stage Great focus differences after objective change Focusing eyepieces are not set correctly. Set condenser correctly, see pages 3-4, 3-5 Correctly click-stop nosepiece Correctly set specimen in specimen holder and fix it. [. . . ]