User manual ZEISS AXIOSKOP

[. . . ] Reflected light bright field (for equipment 0. 3 and 0. 5 only - Axiolab Pol with reflected lighting) . Reflected light polarisation (for equipment 0. 3 and 0. 5 only - Axiolab Pol with reflected lighting) . Assembling and using the overview unit, swivelling for lens 1. 25x (accessory 445313 - for equipment 0. 4 and 0. 5) . Assembly and use of a phase contrast unit (accessory 445303 or 445366) . [. . . ] The other regulations for your safety, as detailed in the instruction manuals, must be observed strictly!· Adjust the lamp helix or light source image as described in the above mentioned instructions. In doing so, orient the lamp housing perpendicular to the stand so that the lamp mount points downwards (no orientation point available). NOTE When using these lamps, the uniformity of illumination for the lens 2. 5x/0. 075 can be improved by slightly readjusting on the focusing screw (symbol with the horizontal double arrow). Check this with a homogeneously reflecting object (mirror), while observing the illumination and the edge of the field. 2 32 09/95 B 40 016 e Axiolab Pol 2. 7. 3 Replacing condensers 1 6 2 5 4 3 Figure 2 25 Replacing the condenser · Raise the rotary stage Pol to the stop by means of the coarse drive (2 25/2). · Lower the condenser carrier by means of the condenser drive (2 25/1) and undo the clamping screw (2 25/3). IMPORTANT When using the overview unit (445313) or the circular polariser (453623), make sure that the rotary bearings of these units do not press on the luminous field diaphragm (2 25/4). · Pull the condenser towards you out of the guide fork; if necessary, tilt down the front optics (shift lever (2 25/5 or 6)). · Insert the other condenser in the reverse order. B 40 016 e 09/95 2 33 Axiolab Pol 2. 7. 4 Fitting and replacing the polariser 1 2 12 13 3 11 10 4 9 14 5 7 8 6 Figure 2 26 Polariser assembly · The swivelling polariser (2 26/5) is secured at two screw on points (2 26/2 and 3) under the condenser carrier (2 26/1). · For installation or removal, use the ball head screwdriver (2 26/6) and a normal screwdriver. For installation, first secure the screw (2 26/4) at the screw in point (2 26/3) and then the screw (2 26/7) at the screw in point (2 26/2). This method requires the following additions: · For equipment 0. 1 to 0. 3: ABBE condenser 0. 9/1. 25 with 5 fold turret disk for bright field, for phase contrast, the ring diaphragms Ph1, Ph2, Ph3 or dark field (0. 65/0. 9) - for the CP Achromat 40x/0. 65 (accessory 445303) · For equipment 0. 4 and 0. 5: for the achr. system condenser 0. 24 Pol (445325) with front optics 0. 9 Pol (445331) the turret disk H D Ph (accessory 445366) for bright field, for phase contrast, the ring diaphragms Ph1, Ph2, Ph3 (accessory 445369 71) if applicable, dark field To do this, use lenses with the additional designation "Ph1, Ph2 or Ph3" (see Section 1. 4 (2); accessories 8 ff. ). · The ABBE condenser 0. 9/1. 25 with turret disk (Figure 2 30) is fitted on the unit as described in Section 2. 7. 3. B 40 016 e 09/95 2 37 Axiolab Pol · After undoing the screw (2 29/1) with the ball head screwdriver (2 29/4), take out the bright field insert (2 29/2) and replace it by the turret disk (2 29/5). · On both condensers, turn the turret disk to the position HF or H by way of the read off index (2 29/8). · The basic settings for transmitted light/bright field detailed in Section 2. 6. 1 are the prerequisite for the following adjustment. · First of all sharply focus the specimen in the bright field and open the luminous field diaphragm (2 25/4) up to the edge of the field. For low contrast specimens, it helps to close the aperture diaphragm (move the slide (2 30/1) or the knurled ring (2 29/7) in the direction of 0. 2 or 0. 3 on the aperture scale). 4 2 3 5 3 6 1 7 8 Figure 2 29 Phase contrast unit for equipment 0. 4 and 0. 5 2 38 09/95 B 40 016 e Axiolab Pol · Swivel in the lens 10x Ph1, if available, and allow the affiliated ring diaphragm Ph1 to engage on the turret disk. · Complete phase contrast is achieved only if the dark phase ring in the lens and the "bright" ring diaphragm are precisely flush (Figure 2 31). · The ring diaphragms in the ABBE condenser are centred by means of the centring screws (2 30/2). NOTE In the case of the system condenser, there are centring screws behind two openings (when the front optical system is swivelled in, before the right hand (2 29/6) or behind the left hand swivel lever). 1 2 3 2 Figure 2 30 ABBE condenser 0. 9/1. 25 Wrong Right · Check the centring of both rings with the BERTRAND lens activated (2 17/1) (equip ment 0. 2 0. 5 - see Section 2. 6. 2 (4)); for equipment 0. 1, with the eyepiece pulled out, at the base of the eyepiece connection or, better, inserted through an auxiliary microscope (accessory 444830) in an eyepiece connection and focused on the ring diaphragm plane. · Adjust the brightness of the lamp accord ing to the specimen. Figure 2 31 Centring the ring diaphragms IMPORTANT More than bright field, phase contrast requires particularly clean glass/air interfaces on the specimen and the optical system (avoid fingerprints). B 40 016 e 09/95 2 39 Axiolab Pol 2. 8 Microphotography / video microscopy / photometry (equipment 0. 4 and 0. 5 only) " symbol). With the aid of the push rod (2 10/4) on the binocular photo tube, the Axiolab Pol microscope can be switched over from visual observation to microphotography/video microscopy(" As this involves 100 % switchover on the photo tube Pol, visual viewing while taking photographs/ video shots is not possible. 3 chip ENG Video zoom adapter Microphotography TV adapter Video microscopy 1 chip C mount 24 5 6 7 8 9 10 11 12 13 14 1 3 Figure 2 32 Connectors for microphotography and video microscopy 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Connector for single lens reflex camera 2. 5x for T2 . [. . . ] 2 29 A 10 09/95 B 40 016 e Axiolab Pol List of abbreviations A a/f AL, A achr. ASTM B BP C, C Mount CB CE CISPR CSA CP d D DC DL, D DF DIC DIN EG EMVG EN ENG Epi EPL E Pl EWG F Fl foc. FWD G HAL HBO HF Aperture diaphragm Opening (across flats) Reflected light Achromatic Aplanatic American Society of Testing Materials Blue glass Bandpass Camera thread Conversion blue (daylight filter) Communautés Européennes (European Communitiy) Comité International Spécial de Perturbation Radioélectrique Canadian Standards Association Clinical Plan (CP Achromat) Specimen thickness Diameter or cover glass thickness Direct current Transmitted light Dark field Differential Interference Contrast German Industrial Standard European Community German law governing the electromagnetic compatibility of devices European standard Electronic News Gathering (bayonet coupling point of CCD cameras) Specially for reflected light Epiplan Plane eyepieces with aspherical corrected optics Europäische Wirtschaftsgemeinschaft (European Economic Community) Field diaphragm, also luminous field diaphragm Fluorescence Focusing Free Working Distance Polarisation optic path difference Halogen lamp Mercury vapour short arc lamp Bright field B 40 016 e 09/95 A 11 Axiolab Pol ICS IEC IF IP ISO l LD LED LP man. XBO Z ZN Inifinity Colour corrected System International Electrotechnical Commission Interference Filter International Protection International Organization for Standardization Wavelength of light Long Distance Light Emitting Diode Long pass Manual Microscope camera Micro hardness tester Motor driven Mikroskop photometer Neutral filter with a transmission of 0. 06 (¢ 6 %) Refractive index of the slow wave Photometry Plane Polarisation Phase contrast Narrowband filter Class of protection Single lens reflex Spectacle wearer Super plane (for photo eyepieces) Technischer Überwachungsverein (German technical inspectorate) Television Universal rotary stage Underwriters Laboratories Visual Xenon short arc lamp Centring ZEISS standard A 12 09/95 B 40 016 e Axiolab Pol References Beyer, H. [. . . ]