User manual ZEISS AXIOLAB POL

[. . . ] Axiolab Pol Microscope Operating manual Unless expressly authorised, forwarding and duplication of this document, utilisa tion and communication of its contents are not permitted. All rights reserved in the event of granting of patents or registration of a utility model. This manual is not covered by an update service. Issued by: Carl Zeiss Jena GmbH Zeiss Gruppe Unternehmensbereich Mikroskopie D 07740 Jena Phone: (03641) 64 2936 Fax: (03641) 64 3144 Number of this Operating manual: B 40 016 e Date of issue: 09/95 Axiolab Pol TABLE OF CONTENTS Page Title page Copyright Table of contents . I IV VI VII IX 1 1. 1 1. 2 1. 2. 1 1. 2. 2 1. 3 1. 4 Description Designation, purpose . 11 11 12 14 15 18 B 40 016 e 09/95 I Axiolab Pol Page 2 2. 1 2. 2 2. 3 2. 4 2. 5 2. 6 2. 6. 1 2. 6. 2 2. 6. 3 2. 6. 4 2. 7 2. 7. 1 2. 7. 2 2. 7. 3 2. 7. 4 2. 7. 5 2. 7. 6 2. 7. 7 2. 8 2. 8. 1 2. 8. 2 2. 8. 3 OPERATION Setting up the unit . [. . . ] · Insert the other condenser in the reverse order. B 40 016 e 09/95 2 33 Axiolab Pol 2. 7. 4 Fitting and replacing the polariser 1 2 12 13 3 11 10 4 9 14 5 7 8 6 Figure 2 26 Polariser assembly · The swivelling polariser (2 26/5) is secured at two screw on points (2 26/2 and 3) under the condenser carrier (2 26/1). · For installation or removal, use the ball head screwdriver (2 26/6) and a normal screwdriver. For installation, first secure the screw (2 26/4) at the screw in point (2 26/3) and then the screw (2 26/7) at the screw in point (2 26/2). · First secure the polariser D, rotary (453620) (2 26/14) at the screw in point (2 26/11) using the key (2 26/8) and then secure the latching rod (2 26/10) at the screw in point (2 26/12). In certain circumstances, slight readjustment analogous to Section 2. 7. 6 may be necessary during the course of replacement. 2 34 09/95 B 40 016 e Axiolab Pol 2. 7. 5 Assembling and using the overview unit, swivelling for lens 1. 25x (accessory 445313 for equipment 0. 4 and 0. 5) For overview observation in a specimen field Ø = 16 mm (when using the Plan Neofluar 1. 25x/0. 04 lens / accessory 440300) in transmitted light bright field or polarisation mode, the above mentioned overview unit is needed to illuminate the field. · To facilitate assembly, detach the rotary stage from the stand as described in Section 2. 7. 1 (3). · Using a screwdriver, first unscrew the fillis ter head screw (2 27/2) on the underside of the polariser D, rotating (2 27/1). · Screw the analogous bearing screw of the large field lens (2 27/4) into the now exposed threaded hole in the rotary bearing of the polariser (use key (2 27/5) to do this). NOTE Re insert the spring washer (2 27/3) on assembly. 1 6 2 3 4 8 5 Figure 2 27 Using the overview unit 7 · Screw on the extended latching rod (2 27/6) analogously to the polariser D, rotating (Section 2. 7. 4) after removing the latching rod (2 27/7). · Insert the condenser as described in Section 2. 7. 3 and raise it by means of the condenser drive (2 25/1). · Position the rotary stage on the stand as described in Section 2. 7. 1 (3) and slide it up until the bottom stop (2 19/3) takes effect, and secure it here. IMPORTANT When a polariser and overview lens are combined, the stage lowering range is limited to 12 mm!In doing so, use must not be made of higher specimens, particularly when carrying out reflected light analyses (equipment 0. 5)!· After adjusting the KÖHLER lighting as described in Section 2. 6. 1 (3), tilt away the front optics of the condenser with the shift lever (2 25/5) and swivel in the overview lens up to the latching position. NOTE In order to use the overview unit, the aperture diaphragm (2 12/5) (see condenser adjustment) must be opened fully!The illustrated luminous field diaphragm is opened up to the edge of the field of vision by means of the setting ring (2 12/4). B 40 016 e 09/95 2 35 Axiolab Pol 2. 7. 6 Assembling the circular polariser D (accessory 453623) · After removing the polariser and, if necessary, the latching rod (see Section 2. 7. 4), the circular polariser D and the extended latching rod can be fitted analogously to the polariser D, rotating. · Prepare the microscope as described in Sections 2. 6. 1, 2. 6. 2 (1) and (2). NOTE Do not use a specimen for the further adjustment steps! · Swivel in the polariser (2 28/3) - bottom flap - up to the latching point and observe extension (dimming of the field of vision without a specimen) at the full light output. If this is not optimum, use a small screwdriver to correct the position of the polariser's oscillation direction by turning slightly and sensitively on the holder - use the adjustment slots (2 28/4) until maximum extension has been achieved. · After removing the dust protection slide, slide the affiliated compensator slide l/4 (2 28/5) into the compensator slot and swivel in the l/4 plate (2 28/2) - top flap - on the circular polariser D. 5 4 1 2 3 2 Figure 2 28 Circular polarisation unit 2 36 09/95 B 40 016 e Axiolab Pol · On the control for the rotary bearing (2 28/1), turn the plate until maximum extinction (dark grey field of vision) is achieved (the lever points 45° to the right). · An (anisotropic) specimen should not be observed until the above mentioned adjustments have been made. Specimens appear constant and, independently of rotation of the stage, in their interference colour, which depends on the material, the thickness of the specimen and its orientation. NOTE For an image rich in contrast, when using higher lens magnification factors (as from approx. [. . . ] (Herausgeber): Handbuch der Mikroskopie Verlag Technik, Berlin 1988 Burri C. : Das Polarisationsmikroskop Birkhäuser Verlag, Basel 1950 Burri C. : Zur Theorie und Praxis der Drehkompensatoren nach BEREK und EHRINGHAUS Z. 4, 418 - 426, 1953 El Hinnawi: Methods in Chemical and Mineral Microscopy Elsevier Publishing Company, Amsterdam London New York 1966 Emons, H. H. : Chemische Mikroskopie Deutscher Verlag für Grundstoffindustrie, Leipzig 1973 Freund, H. (Herausgeber): Handbuch der Mikroskopie in der Technik - 8 Bände Umschau Verlag, Frankfurt am Main 1954 ff. [. . . ]