User manual ZEISS AXIOLAB A

[. . . ] Axiolab A Reflected-Light Microscope Operating Manual Carl Zeiss Axiolab A Knowledge of this instruction manual is necessary for device operation. Please get familiar with its contents and especially the precautions for safe device operation. Changes due to further technical development are reserved; this manual is not covered by an update service. © Unless expressly authorized, forwarding and duplication of this document, utilization and communication of its contents are not permitted. [. . . ] 440 mm observation and camera/video port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . tube factor 1× observation and camera/video port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100%/0% or 0%/100% camera/video port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60 mm interface Eyepieces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . plug-in diameter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 mm B 40-015 e 06/99 1-23 Carl Zeiss Axiolab A 1-24 06/99 B 40-015 e Axiolab A Carl Zeiss START-UP Contents 2 2. 1 2. 2 2. 3 2. 3. 1 2. 3. 2 2. 4 2. 5 2. 6 2. 6. 1 2. 7 2. 8 2. 9 Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3 Unpacking the Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3 Screw in Objectives. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3 Insertion of Eyepieces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4 Insertion of Eyepiece Reticle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4 Compensation of Ametropia when Eyepiece Reticles are used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5 Setting of Interpupillary Distance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5 Attachment of Reflected-Light Halogen Illuminator. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6 Retrofit the Transmitted-Light Halogen Illumination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6 Switch on the Transmitted-Light Halogen Illuminator. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7 Equipment of Filter Slider . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7 Set the Luminous-Field Diaphragm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8 Connecting the Instrument to the Line . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8 B 40-015 e 06/99 2-1 Carl Zeiss Axiolab A 2-2 B 40-015 e 06/99 Axiolab A Carl Zeiss 2 START-UP The three versions of the Axiolab A reflected-light microscope, including accessories, are supplied in standard packaging. We would recommend you to keep the transport cases for storage or return of the instrument to the manufacturer. 2. 1 Unpacking the Instrument · Remove the microscope from the transport case and place it on the mat (2-1/1) provided on the worktable. · Remove the adhesive strips which secure the various controls during transport. · Leave dust protection and technique sliders (2-1/2, 5, 6) inserted in the reflected-light illuminator H (2-1/3) in the instrument. Fig. 2-1 Unpacking the instrument 2. 2 Screw in Objectives · Remove the dust caps (2-2/2) according to the number of objectives and screw the objectives into the nosepiece (2-2/1) clockwise one by one, starting with the lowest magnification (2-2/3). The dust caps should remain on those nosepiece eyes which are not required. Fig. 2-2 Screw in objectives B 40-015 e 06/99 2-3 Carl Zeiss Axiolab A 2. 3 Insertion of Eyepieces · Remove both protection caps from the binocular tube. E-PL 10×/20 Br. , in one tube and the focusing eyepiece E-PL 10×/20 Br. The focusing eyepiece is used to compensate for ametropia of the user's eyes. 2. 3. 1 Insertion of Eyepiece Reticle The E-PL 10×/20 Br. eyepieces are intended for use with eyepiece reticles (see overview under 1. 6). The slight image shift caused by the additional path through the glass is taken into account on the diopter scale by the fact that the zero point position is indicated not by the white dot (2-3/W) but by the red dot (2-3/R). The eyepiece reticles (2-3/1) have been adhered to screw-in mounts (2-3/2) to allow easy replacement. Fig. 2-3 Insertion of eyepiece reticle · To replace a reticle, unscrew the screw-on mount containing the eyepiece reticle and replace it with the required one. If eyepiece reticles are inserted into the unscrewed mount by the customer, attention must be paid to the labelling being visible the right way up after insertion. 2-4 B 40-015 e 06/99 Axiolab A Carl Zeiss 2. 3. 2 Compensation of Ametropia when Eyepiece Reticles are used The correct use of an eyepiece reticle requires two focusing eyepieces, e. g. · Use the eyelens of the focusing eyepiece to focus on the line figure of the eyepiece reticle; focus on the edge of the field of view if no eyepiece reticle is used. · Focus on the microscope image of a specimen via the focusing drive by looking through the eyepiece with reticle. · When the image and the eyepiece reticle are in focus in the above eyepiece, focus the image for the second eye via the focusing eyelens of the second eyepiece. The position of the focusing drive on the stand must not be changed. 2. 4 Setting of Interpupillary Distance · The eyepiece distance is matched to the individual interpupillary distance by swinging the eyepiece tubes symmetrically towards one another. The eyepiece distance can be set in the range between 55 and 75 mm, and you should try to remember the individual value once set. B 40-015 e 06/99 2-5 Carl Zeiss Axiolab A 2. 5 Attachment of Reflected-Light Halogen Illuminator · Attach dovetail guide (2-4/3) of 6 V, 30 W halogen lamp (2-4/4) to the microscope stand and tighten hexagonal screw (2-4/2) using the SW 3 screwdriver (2-4/1). [. . . ] When using the Axiolab A in humid climatic zones, proceed as follows: Store the Axiolab A in bright, dry and well ventilated rooms with a humidity of less than 85%; store particularly sensitive components and accessories, such as objectives and eyepieces, in a dry closet. When the equipment is stored in closed cases for a longer period of time, the growth of fungus can be avoided by including cloths soaked in fungicide in the cases. - - The risk of growth of fungus on opto-mechanical instruments always exists in the following conditions: - relative humidity of more than 75% and temperatures between +15° C and +35° C for more than three days. installation in dark rooms without air ventilation, and dust deposits and fingerprints on optical surfaces. - B 40-015 e 06/99 4-3 Carl Zeiss Axiolab A 4. 2 Troubleshooting Troubleshooting on the Axiolab A microscope is limited to only a few actions: · Checking the line cable · Checking the set instrument voltage and changing the fuses · Checking the illumination and changing the lamp - Defective fuse(s) change the fuses according to paragraph (1) - defective halogen lamp change the lamp according to paragraph (2) or (3) (1) Check the Line Cable and Fuses and Change Fuses Make sure to disconnect the instrument from the line first!· Use 2. 0 mm watchmaker's screwdriver to press the two springs at the side of the fuse holder (4-1/2) to the inside via the groove and remove fuse holder. 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