User manual MOTIC LIVE IMAGING MODULE

[. . . ] USB 2. 0 driver and DirectX9. 0b (or higher version) have been correctly installed. Please confirm the destination path, and click "Next". Click "Continue Anyway" to continue. Files will be copied to your computer. 3 Click "Finish". User Interface The Motic Live Imaging Module contains Title bar, Control Panel, Image Preview Window and Status Bar. Click one of the following four buttons to display the corresponding control panel: Basic Adjustment panel; 4 Color Adjustment panel; Advanced Setting panel; Video Capture panel. Image Preview Window: Displays the real-time live image coming from your Moticam or Digital Microscope. Status Bar: Shows the current frame rate you can increase the frame rate by increasing illumination and decreasing the exposure rate, or by lowering the live resolution on the title bar to hide this window, re-click to display it on the title bar to hide the control panel, re-click to Basic Operation of Motic Live Imaging Module Language Setting From the Language dropdown list, select a language of your choice. 5 Device Setting & Image Preview Connect an imaging device to your computer and open this image Module, the connected device will be auto-opened and the live image will display. [. . . ] If you wish to override this, use the slider to adjust the settings manually. 8 The Background Balance function will help reduce the effects of uneven illumination. To use it properly, please setup your microscope, focus on your slide and adjust the microscope's illumination and the Exposure settings above accordingly. Next move the slide away from the camera's Field of View and click on the Background Balance button. You can adjust exposure settings if you wish and also perform the White Balance function below. Note: When you change the microscope's objective or adjust the microscope's illumination intensity, please perform the above steps again. 9 The "White Balance" button will help get the best Color similar to what you see through the eyepieces. Setup your microscope properly, focus on your slide, then remove the slide from the Field of View and click on the White Balance button. Note: Some imaging chips will have an automatic hardware white balance. You will see the white balance being adjusted automatically as close to the real color as possible. In order to adjust the color even closer, please use the tools in the Color Adjustment Panel. Note: Each "Apply" checkbox will be auto checked while clicking the "Background Balance" and "White Balance" buttons. Recheck it to cancel the corresponding function. You can also adjust the gain, offset and other values by dragging the corresponding slider bar to improve the image quality; if the image is displayed upside down, check "Mirror" or "Flip" to correct it. 10 2. Color Adjustment Click the button to display the Color Adjustment panel, in which you are able to do some color adjustments to the image if necessary. Color Correction Click on "Enable" to activate this function. Then drag the slider bar to a suitable setting for the intensity of the correction. This function was designed to distinguish between Blue, Red and Purple and will enhance in that spectrum. This is useful especially for H&E stained specimens. RGB Adjustment For full manual color control, you can adjust both Gain and Brightness of a specific color separately. To go back to the default camera settings, click on "Reset". 11 You can display a color Histogram by clicking on the button. This will show the pixel gray scale distribution of the entire image or a user defined ROI area. By default, the Histogram window displays the histogram of the entire image. Right click within the window and select the "ROI Histogram" command to display the histogram of the user defined ROI area, click the command again to cancel it. the command "ROI 12 Advanced Setting Click the button to set advanced settings. From the Filter Setting group box, check the corresponding checkbox to enable the function of Filter, Edge Detection or Sharpness. [. . . ] Users are able to save and load the parameter settings that have been set within the Basic Adjustment panel, Color Adjustment panel, and Advanced Setting panel. Note: A name should be input in the blank before saving the corresponding parameter settings. 15 Video Capture Click the button to display the Video Capture panel, with which you are able to capture images and record videos. 1. Image Capture Select a size at which to capture an image from the Format dropdown list. [. . . ]