User manual LEICA TCS SP5 II BROCHURE TECHNICAL DATA

[. . . ] Leica TCS SP5 II The Only Broadband Confocal Technical Documentation Living up to Life Specifications Upright Leica DM6000 CS Leica DM6000 CFS Leica DMI6000 CS Leica DMI6000 CS bottom port For imaging Passive 1500 m travel range/3 nm stepsize Travel range depending on mechanics of microscope/15 nm step size For imaging WLL, average power 1. 5 mW: 470­670 nm Diode, 40 mW: 442 nm Ar, 65 mW: 458, 476, 488, 496, 514 nm HeNe, 1 mW: 543 nm HeNe, 2 mW: 594 nm HeNe, 10 mW: 633 nm DPSS, 20 mW: 561 nm UV OPSL, 80 mW: 355 nm UV Laser type IR Diode, 50 mW: 405 nm For imaging TiSa (ps or fs) 1 W 690. . . 1040 nm (various ranges) ­ ­ ­ For imaging Up to 8 channels Up to 3 channels Yes Optional Microscopes Inverted Specification Microscope anti-vibration table Vibration insulation SuperZ galvanometer stage Z-drive Motorfocus (stand) Laser type VIS Continuous wave lasers Pulsed lasers VIS UV Modulation type AOTF VIS Excitation modulation AOTF UV EOM IR Pulsed laser driver 2 Specifications Features Number of laser ports Number of lasers Excitation ­ emission splitting Detection range UV and IR imaging Field upgradable For imaging Up to 3 (UV - VIS - IR) Up to 8 Acousto Optical Beam Splitter (AOBS®) or dichroic beam splitters 400. . . 800 nm Sequential or simultaneous Yes (UV, IR) Individual precise correction optics (up to 5 positions) Alignment stable single pinhole Motorized by software, automatic mode available for DM6000/DMI6000: available for DM6000 CFS: not available 458 / 514 nm 488 / 561 / 633 nm For imaging Optically correct scanning at low inertia Conventional and resonant scanner in one system (optional) For imaging (PMT and APD) 2800 Hz 1 Hz 1400 5 Hz 50 Hz Yes 8192 x 8192 pixel 1. 0 . . . [. . . ] grey resolution Read out frequency Detection features Emission separation Confocal channels Sensors External confocal detection Quantum efficiency Dark counts Jitter FWHM Dead time Detection types Non-confocal detection Transmitted light detector Non descanned transmitted light channels Non descanned reflected light channels Devices Scanner control Trigger in/out functions Auxiliary data input channels Electronics Max channels in parallel Computer Monitors Integration of third party software ­ 5 Specifications Devices For imaging Optional Optional Various options Intuitive and guiding user interface Included Included Included Included Included Included Included Included For imaging Interactive data recording also allowing job sequencing and online evaluation Combines Mark & Find with sophisticated 3D recordings, Live Data Mode etc. Maximum and other projections, simulated fluorescence process, rotation animations, stereo pairs, red-green anaglyphs, height color coded extended depth of focus images etc. Histogram based colocalization and area measurements Deconvolution option for widefield and confocal images FRAP wizard, FRAPxt wizard, FLIP wizard, FRET SE wizard, FRET AB wizard etc. ­ ­ Interactive data recording also allowing correlation of optical and electrical data Extensions Fast ROI-spectrometer Auxiliary emission port Environment accessories General Context sensitive online help system Multi-dimensional data acquisition Region of interest (ROI) scan Software (LAS AF) Excitation line/frame sequential scan Emission spectrum recording Quantification tools Multi-color restoration, spectral unmixing General time lapse experiment control tile scanning (mosaic scan) Dedicated application wizards Live Data Mode Advanced Mark & Find 3D visualization Software options (LAS AF) Colocalization Deconvolution MicroLab SMD FCS wizard SMD FLIM wizard Electrophysiology 6 9 1 2 11 12 6 13 14 7 10 5 8 4 3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Research Microscope Scanhead Workstation Microscope Control Unit Laser Supply and Power Supply Control Panel Computer Table Anti-vibration Table Monitor Supply Control Smart Move EL6000 Fluorescence Illumination Keyboard Computer Mouse 7 Installation Requirements Weight base system: visible and ultraviolet radiation: VIS: max. 100 kg VIS: 3, 2 kW UV: 0, 5 kW IR: 1. 5 kW UV laser, air-cooled heat exchanger IR laser, air-cooled heat exchanger (chiller) VIS lasers: 100 . . . 240 V AC ± 10 % 2 x 1600 VA, 50/60 Hz (Power input 1+2) UV laser: 100 . . . [. . . ] + 25 °C Avoid proximity to air conditioning equipment Protect from dust Room darkening recommended [. . . ]