User manual HANNA INSTRUMENTS HI 83746

[. . . ] 5 PRINCIPLE OF OPERATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 ABBREVIATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 FUNCTIONAL DESCRIPTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 GUIDE TO DISPLAY CODES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [. . . ] Phenols interfere in the Fehling reaction and therefore red wine must be decolorized prior to analysis. Wine also contains non-fermentable reducing sugars like pentose which will also be analysed by this method. Typical content of reducing sugars in must and wine sweet must 20-25 % Must normal 10-20 % in fermentation 4-12. 5 % Wine Sweet Semi sweet Almost dry Dry 2. 5-12. 5 0. 8-2. 5 0. 2-0. 8 0-0. 2 % % % % 4 200-250 g/L 100-200 g/L 40-125 g/L 25-125 8-25 2-8 0-2 g/L g/L g/L g/L BATTERIES REPLACEMENT Battery replacement must only take place in a nonhazardous area. The blinking " " will appear when the batteries power gets low. When batteries are completely discharged, "0% bAtt" will appear and after two seconds the instrument is switched off. Remove the battery cover from the bottom of the instrument and change the old batteries with 4 fresh 1. 5V batteries, paying attention to the correct polarity. Replace the cover. ACCESSORIES REAGENT SETS HI 83746-20 Reducing Sugars reagents set (ca. 20 tests) HI 93703-59 Charcoal for decoloration of Red Wine (about 100 tests) OTHER ACCESSORIES HI 839800 Reactor HI 740216 Test tube cooling rack (for 25 vials) HI 740217 Laboratory bench safety shield HI 731331 Glass cuvets (4 pcs) HI 731340 200 µL automatic pipette HI 731350 Plastic tips for 200 µL automatic pipette (25 pcs) HI 731341 1000 µL automatic pipette HI 731351 Plastic tips for 1000 µL automatic pipette (25 pcs) HI 740232 Filter paper type I (100 pcs) HI 731325W Caps for cuvets (4 pcs) HI 93703-50 Cuvets cleaning solution (230 mL) HI 740027P 1. 5V AA batteries (10 pcs) HI 710005 Voltage adapter from 115V to 12 VDC (USA plug) HI 710006 Voltage adapter from 230V to 12 VDC (European plug) 17 If the expected RS concentration is less than 5. 00 g/L please refer to the instructions below: · Preheat the Hanna Reactor HI 839800 to 105 °C (221°F). For a correct use of the reactor follow the Reactor Instruction Manual. · Remove the cap from two vials HI 83746A-0 Fehling Solution A. SPECIFICATIONS Range Resolution Precision Light Source Light Detector Method Environment Battery Type Dimensions Weight 0. 00-50. 00 g/L 0. 25 g/L ±0. 50 @ 10. 00 g/L Tungsten lamp with narrow band interference filter @ 610 nm Silicon Photocell Fehling Method 0 to 50°C (32 to 122°F); max 95% RH non-condensing 4 x 1, 5 volt AA batteries / 12 to 20 VDC through voltage adapter 224 x 87 x 77 mm (8. 7 x 3. 3 x 3. 1") 512 g (17, 6 oz. ) 1 mL 1 mL · Use the HI 731341 1000 µL automatic pipette to add exactly 1 mL (=1000 µL) of HI 83746B-0 Fehling Solution B to each vial. For a correct use of the automatic pipette please follow the related Instruction Sheet. wine sample 1 mL deionized water 1 mL REQUIRED REAGENTS Code Description HI 83746A-0 Fehling solution A HI 83746B-0 Fehling solution B OPTIONAL REAGENTS Code HI 93703-59 Quantity/test 1 vial 1 mL · Use the HI 731341 1000 µL automatic pipette to add exactly 1 mL (=1000 µL) of wine sample to one vial (Sample) and 1 mL of deionized water to the other vial (Blank). Description Charcoal Quantity/test 2 spoons Sample Blank PRECISION AND ACCURACY · Replace the cap and invert the vials several times to mix. · The concentration displayed on the LCD must then be divided by 4 to obtain the correct value in g/L (ppt) of Reducing Sugar. Accuracy is defined as the nearness of a test result to the true value. Although good precision suggests good accuracy, precise results can be inaccurate. In a laboratory using a standard solution of 10. 00 g/L of Reducing Sugars and a representative lot of reagent, an operator obtained with a single instrument a standard deviation of ±0. 50 g/L. 5 16 PRINCIPLE OF OPERATION Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices. If pure absorption occurs, the fraction of light absorbed depends both on the optical path length through the matter and on the physical-chemical characteristics of the substance according to the Lambert-Beer Law: -log I/Io = c d or A = c d Where: -log I/Io Io I c d = = = = = = Absorbance (A) intensity of incident light beam intensity of light beam after absorption molar extinction coefficient at wavelength molar concentration of the substance optical path through the substance · After a few seconds the display will show "-0. 0-". Remove the Blank Vial. · Insert the Sample Vial into the instrument. Note: If the sample vial appears brown/orange without blue hue, dilute the wine sample and repeat the procedure. Note: This operation is necessary to recover the condensed water after heating. When the LCD displays "---", it is ready. A microprocessor controlled special tungsten lamp emits radiation which is first optically conditioned and beamed to the sample contained in the cuvet. [. . . ] · Whenever the vial is placed into the measurement cell, it must be dry outside, and completely free of fingerprints, oil or dirt. Wipe it thoroughly with HI 731318 or a lint-free cloth prior to insertion. DIGESTION · Use of the optional HI 740217 safety shield is recommended. At the end of the digestion period, the vials are still hot: allow the vials to cool to room temperature in the optional HI 740216 test tube cooling rack. [. . . ]