User manual HANNA INSTRUMENTS HI 83215

[. . . ] Instruction Manual HI 83215 Grow Master Basic for Nutrient Analyses www. hannainst. com 1 Dear Customer, Thank you for choosing a Hanna product. Please read this instruction manual carefully before using the instrument. This manual will provide you with the necessary information for the correct use of the instrument. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst. com. TABLE OF CONTENTS PRELIMINARY EXAMINATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [. . . ] The nutritive elements are differently absorbed by the plants, hence the nutrient solution loses substances, becomes impoverished and must be enriched. High range normally corresponds to the typical values of nutrients solutions. It is therefore possible to verify that the solution given to the plants contains the correct quantities of nutritive substances. 18 SAMPLE PREPARATION SAMPLE PREPARATION PROCEDURE Note: If the water sample to be analyzed is very turbid, let it stand in a beaker for a while until most of the solid particles have settled. Then, use the pipette to transfer the supernatant solution to the other beaker and prepare the samples as described below. To prevent the displacement of the settled solids at the bottom of the beaker, do not induce air bubbles into the solution. IRRIGATION WATER (LR): · Measure 100 mL of sample with the graduated cylinder. · If the solution contains some turbidity or color, pour it in the large 170 mL beaker and add a powder packet of active carbon. · Mix well using the spoon and then wait for 5 minutes. 19 · Fold a filter disc twice as shown in the figure. Insert the folded filter disc in the funnel. · Filter the treated sample into an empty beaker. The sample is now ready. Note: Filter at least 40 mL of solution if all the four methods will be tested. If the solution is still turbid or colored, treat it again with a packet of active carbon. After use, throw the filter disc away and wash the syringe and the filter assembly well. NUTRIENTS SOLUTION (MR): · Use the graduated cylinder to measure exactly 20 mL of sample. · Remove the cap and fill the Demineralizer Bottle with tap water. 20 · Replace the cap and shake gently for at least 2 minutes. · Open the upper part of the Demineralizer Bottle cap and gently squirt the demineralized water into the cylinder, up to the 100 mL mark. Note: The ion exchange resin contained in the Demineralizer Bottle is provided with an indicator substance. The indicator will change from green to blue when the resin has been exhausted and needs to be replaced. · Pour the solution in the large 170 mL beaker, replace the cap and invert several times to mix. · If the solution contains some turbidity or color, add a powder packet of active carbon and follow the procedure described for Irrigation Water (LR). NUTRIENTS SOLUTION (HR): · Add 10 mL of sample to the graduated cylinder using the 5 mL syringe (twice). 21 Note: To measure exactly 5 mL of sample with the syringe, push the plunger completely into the syringe and insert the tip into the sample. Pull the plunger out until the lower edge of the seal is on the 5 mL mark of the syringe. · Remove the cap and fill the Demineralizer Bottle with tap water. · Replace the cap and shake gently for at least 2 minutes. · Open the upper part of the Demineralizer Bottle cap and squirt gently the demineralized water into the cylinder, up to the 100 mL mark. · Pour the solution in the large 170 mL beaker, replace the cap and invert several times to mix. · If the solution contains some turbidity or color, add a powder packet of active carbon and follow the procedure described for Irrigation Water (LR). 22 AMMONIA HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0 to 100 mg/L 1 mg/L ±1 mg/L ±4% of reading at 25 °C ±1 mg/L Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92, Nessler method. The reaction between ammonia and reagents causes a yellow tint in the sample. Description Quantity First Reagent 4 drops (6 drops for seawater) Second Reagent 4 drops (10 drops for seawater) REQUIRED REAGENTS Code HI 93715A-0 HI 93715B-0 REAGENT SETS HI 93715-01 Reagents for 100 tests HI 93715-03 Reagents for 300 tests For other accessories see page 50. MEASUREMENT PROCEDURE Note: for sample preparation follow the NUTRIENTS SOLUTION (HR) procedure at page 21. · Select the Ammonia HR method using the procedure described in the Method Selection section (see page 12). · Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap. The display will show "-0. 0-" when the meter is zeroed and ready for measurement. 10 mL 23 Ammonia HR · Remove the cuvette. · Add 4 drops of HI 93715A-0 First Reagent (6 drops for seawater analysis). [. . . ] · Fill the cuvette with 10 mL of sample, up to the mark. 10 mL · Add 6 drops of HI 93750A-0 Potassium Reagent, replace the cap and swirl the solution. · Place the cuvette into the holder and close the lid. Potassium LR 46 · Press the Zero key. The display will show "-0. 0-" when the meter is zeroed and ready for measurement. · Remove the cuvette and add the content of one packet of HI 93750B-0 reagent. Replace the cap and gently mix for one minute by slowly turning the cuvette upside down. · Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 2 minutes and press Read. [. . . ]